Libraries are generated and sequenced and 10x Barcodes are used to associate individual reads back to the individual partitions. It does so by partitioning thousands of cells into nanoliter-scale Gel Beads-in-emulsion (GEMs), where all generated cDNA share a common 10x Barcode. A pool of ~750,000 barcodes are sampled separately to index each cell’s transcriptome and cell surface protein. This is accomplished by labeling cell surface proteins with antibodies conjugated to a Feature Barcode oligonucleotide, followed by direct capture of the Feature Barcode by the Gel Bead primer. The Chromium Single Cell Feature Barcode technology offers a comprehensive, scalable approach to detect cell surface proteins along with the gene expression and immune repertoire information from the same single cell.
0 Comments
Leave a Reply. |